International Journal of
TechnoChem Research

ISSN 2395 - 4248

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NEWS & UPDATION

  • 2017, Volume 3 No 3 of International Journal of TechnoChem Research is released.
  • 2017, Volume 3 No 3 of International Journal of TechnoChem Research is released.

    2017, Volume 3, No 3, of International Journal of TechnoChem Research is released

  • Included in CAS Database, USA [by Chemical Abstracts Service, USA]
  •  International  Journal of TechnoChem Research

    has been selected for inclusion in CAS,USA  by  Chemical Abstracts Service, USA.

  • International Journal of TechnoChem Research
  • International Journal of TechnoChem Research has been selected for inclusion in CAS,USA by Chemical Abstracts Service, USA.

    International Journal of TechnoChem Research has been selected for inclusion in CAS,USA by Chemical Abstracts Service, USA.

ABSTRACT

Screening of phosphate solubilizing fungi from Rhizosphere soil and study on its alkaline phosphatase activity

Rathi Devi P and Packialakshmi N

ABSTRACT

Abstract : Soil is a mixture of organic nutrients, which provides nutrients for plant growth. Plants require N, P & K for their growth and vegetative propagation. Among vital nutrients, Phosphorus is less available to plants and microorganisms aid in phosphate solubilization. The present study is an ecofriendly method to induce phosphate solubilization in Rhizosphere soil. Totally 25 isolates were isolated from rhizosphere using Pikovskaya mediumand screened for phosphate solubilization using Tricalcium phosphate supplemented minimal medium.Of these, Aspergillus sp and Penicillium sp were found to produce remarkable solubilization index. Hence these two isolates were used for further assays. Enzyme assay was carried out to determine the enzyme activity using cell free extractsand the activity was analyzed by spectrophotometry.In addition, HPLC also performed to determine the end products of phosphate solubilization.The results of HPLC studies revealed that tricalcium phosphate was solubilized into organic acids like citric acid, gluconic acid and malic acid. The results of present study are an evidence for the production of organic acids by Aspergillus sp and phosphate solubilization which induce crop improvement as well as sustainable agriculture. Keywords : Fungi, Phosphate solubilization, Enzyme assay, HPLC, Organic acids

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