International Journal of
TechnoChem Research

ISSN 2395 - 4248

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NEWS & UPDATION

  • Released Released 2016, V2N1 issue of International Journal of TechnoChem Research
  • 2016 Volume 2, No 2 of International Journal of TechnoChem Research is released.

    2016 Volume 2, No 2, of International Journal of TechnoChem Research is released

  • Included in CAS Database, USA [by Chemical Abstracts Service, USA]
  •  International  Journal of TechnoChem Research

    has been selected for inclusion in CAS,USA  by  Chemical Abstracts Service, USA.

  • International Journal of TechnoChem Research
  • International Journal of TechnoChem Research has been selected for inclusion in CAS,USA by Chemical Abstracts Service, USA.

    International Journal of TechnoChem Research has been selected for inclusion in CAS,USA by Chemical Abstracts Service, USA.

ABSTRACT

Curcumin Complex-DNA Interaction Studied By Fourier Transform Infrared Spectroscopy

K.Senthil,  P.M.Andavan

ABSTRACT

Curcumin is a non toxic, a dietary natural polyphenol known for its potent anti-inflammatory, antioxidant, antimutagenic and anticarcinogenic effects. The interaction of curcumin Complex(Curcumin and metal ions) with DNA is widely used in clinical treatment of a variety of neoplastic diseases, but its therapeutic effects is not fully understood. We here report the binding mechanism and structural properties of curcumin with DNA considered as a therapeutic target. The interaction of DNA with curcumin is investigated in aqueous solution at physiological pH with durg/DNA molar ratios of r=1:40, 1:20, 1:10, 1:5 and 1:2. Fourier Transform Infrared(FT-IR) and UV-absorption spectroscopy are used to determine the secondary structure of drug and DNA, drug binding sites, binding constant, as well as the structural variations of curcumin-DNA complexes in aqueous solution. Our spectroscopic result showed that interaction occurred mainly through G-C ,A-T base pairs and major interaction with phosphate groups with overall binding constant of K= 2.95×105 M-1. Curcumin complexes maybe induced biopolymer conformational changes with DNA in the presence of metal ions. Keywords: DNA, Curcumin; FT-IR spectroscopy.

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